Rotavirus and coronavirus are the two most common viral causes of neonatal calf diarrhea and their presence causes a lot of economic damage to the farmers as well as suffering to the animal. By getting better knowledge about the viruses we may help in tracing transmission and in producing new vaccines. This paper served to study the molecular epidemiology of rotavirus and coronavirus in fecal samples collected from two different farms in the state of São Paulo, Brazil. These samples have been studied with focus on the gene VP4, VP7, NSP4 in rotavirus and the gene S1 in coronavirus. From the first farm the screening PCR showed that 12/48 samples were positive for rotavirus and 4/48 positive for coronavirus.

The following project has been made in collaboration with, Denator AB, a biotechnology enterprise. One-dimensional electrophoreses have been used to analyze protein contents of tissue samples. Tissue samples have been either treated according to the company´s newly developed tissue treatment technique or not treated at all and comparisons between these samples have been made. In order to see differences in protein patterns between samples more clearly, a conversion method has been developed where electrophoresis gel visual patterns are used to produce curves, similar to densitometer curves.Denator AB would like to know if the proteins in their treated samples will change visibly over 48 h when incubated at room temperature. This has been investigated using the above mentioned method.

Toxic metals contaminate soil worldwide and thus serve as sever environmental threat. Therefore the purposes of this study were to investigate in which soil fractions that different heavy metals (Fe, As, Cu, Zn and Pb) could be found in contaminated soils and if it is possible to use sieving as a method for decontamination. Soil samples were collected from three different locations, the Nasa silver mine, the Blaiken-mine and Svalget environmental station. The samples were oven dried and later on sieved into six different fractions 8mm, 4 mm, 0.5 mm, 0.250mm, 0.063 mm and <0.063 mm. The fractions 4 mm, 0.5 mm and <0.063 mm from each location were analyzed in an x-ray fluorescence detector.

In the pulp industry, digesters are used to disolve lignin in wood chips. The concentration of lignin is measured and is called the Kappa number. In this thesis, the question of whether an online Kappa sensor, taking samples from the mid-phase of the digester, is useful or not is analyzed. For the samples to be useful, there has to be a relationship between the measured Kappa at the mid- phase and the measured Kappa in the blowpipe at the bottom of the digester. An ARX model of the lower part of the digester has been estimated.

Infection with the bovine lungworm - Dictyocaulus viviparus - is occasionally a problem in Swedish cattle herds. The infection could act in synergism with other infections such as Corona virus to create a poorer welfare and productivity in cattle. Studies have shown that a diagnosis can be made with both the Baermann method and with an ELISA. In Norway diagnosis of BVDV on a herd level is attained through pooled serum samples. The question was raised whether pooled serum samples could be used in a similar fashion to attain status on a herd level for lungworm.

This paper deals with the embalming process in ancient Egypt. Samples were collected from six objects from Medelhavsmuseet in Stockholm. The objects were one supposed ladle and five different contents from pottery. Fourier transform infrared spectrometry (FTIR) and gas- chromatography/mass spectrometry was used to characterize the components of the materials. The result was then correlated with previously made analysis of embalming materials to discover similarities.

The flagellate Giardia intestinalis has been known to cause diarrhea in both man and dog. Results from epidemiological studies have indicated that transmission of the parasite from dog to man is plausible even though that route of infection has yet to be shown. Several international studies have shown that the parasite frequently occurs in asymptomatic dogs. In Sweden the prevalence of Giardia in healthy dogs is not known though. In this study, faecal samples were collected at two separate occasions from 21 litters and 21 bitches showing no symptoms from the gastrointestinal tract. The samples from each litter were pooled but the samples from the bitches were analyzed individually.

A sample of milk inoculated with S. aureus was treated with penicillin, and Antipen (a new commercial product containing penicillinase) was added. To another sample with S. aureus and penicillin, formic acid to pH 5 was added, and to yet another sample, a combination of formic acid and Antipen was added. Controls with no penicillin were treated in the same way.

Babies born prematurely are deprived of normal sensorimotor stimulation when placed in an artificial environment, the incubator. The aim of this project was to design a series of products that could promote the baby?s emotional and neurological development, and to facilitate parental bonding. The products designed in this project has a theoretical basis on the research available today on cognitive and emotional development in children born prematurely. They were developed in close cooperation with a group of parents and nurses in a neonatal intensive care unit.Presence is a product collection for developmental care, consisting of a mattress, a figure and a necklace. The mattress has vibration sensors that transfer the mother?s heart beat and speakers with recorded heartbeat and her voice.

The freshwater pearl mussel Margaritifera margaritifera is an endangered species. To survive, the young mussels demand a fairly coarse bottom material containing an appropriate quantity of organic substrate to survive without suffocating. In order to estimate if a bottom is suitable for juvenile mussels, samples of the sediment are needed. To obtain samples a sampling device called the Mulv collector has been developed within this project. Bottom samples were collected from 18 stations in 4 south Swedish streams, Vramsån, Kling-storpsbäcken, Brönnestadsån and Bräkneån.

Quality is the main focus in management of all laboratories. Accurate results of the analyses are not only determined by the analytical procedure but also by preanalytical factors. In the total analytical process of clinical specimens, there are many possible preanalytical sources of error. Monetoring of temperature on test samples of the transport boxes is one way to reduce the mistakes in the preanalytical phase.In this study, four laboratories from primary health care were invited to participate. The temperature has been measured on test samples of the transport boxes being delivered to the laboratory.In three cases the temperature remained within the limits, but in the fourth case the temperature varied more than the allowed interval.

Cryptosporidium parvum is a protozoan parasite causing diarrhoea in many different animal species including cattle and man. It is an important enteric pathogen in neonatal calves and it is the second most common pathogen found in diarrhoeic calves in Sweden. Subclinically infected adult cattle have, in international studies, been shown to shed a low number of oocysts in faeces and this has been recognised as a potential source of infection for new-born calves. The detection methods used for diagnostic purposes are based on microscopic investigation of faecal smears. These methods have a fairly low sensitivity and samples from subclinically infected cattle have to be concentrated before analysis.

When grains of wheat and rye are conventionally milled, large quantities of bran classified as by-products are left and are mainly used for animal feed. Bran is a complex material composed of the aleurone layer, nucellar epidermis, a seed coat, a fruit coat and a small proportion of the germ and endosperm depending on extraction rate. The bran fraction consists of approximately 40-50 % dietary fibre which can contribute to increase the nutritional quality of human cereal food. It would also be valuable for the food industry to convert by-products as bran to products with higher commercial values. The aim of this thesis was to analyse the content and composition of dietary fibre of heat treated wheat and rye bran according to the Uppsala method with some modifications in order to analyse soluble and insoluble dietary fibre separately. Half of the bran samples were precooked, dried and roasted and the other half dried and roasted (uncooked).

High levels of ammonia in grass silage have been up for discussion by Swedish advisers. One reason is that it is more difficult for the rumen-microbes to utilise high levels of ammonia. A low level of ammonia is also good because if the cattle can use more of the protein in the silage, they don?t need so much protein concentrate. That is very good for the economy at the farm, and it is also good for the environmental problems caused by high levels of ammonia. In this project, 24 samples of silage have been taken at 20 different farms in the south west of Sweden.

With this essay we want to enlighten the problems to match the brand image an enterprise has with their determination of prices. The purpose with this study is to examine how customers apprehend when cloth enterprises extend their brand in the same category. Further we want do identify how this kind of brand extension can affect the position on the market the enterprise has. Thereby we want from incumbent relevant theories develop factors which enterprises ought to take into consideration at the time of a brand extension in the same category..